枯草芽孢杆菌对绵羊瘤胃上皮细胞β-防御素-1表达的影响

doi:10.11843/j.issn.0366-6964.2015.05.011

Abstract

Abstract:

In this study，Bacillus subtilis was selected to investigate its effect on the β-defensin expression of ovine ruminal epithelial cells cultured in vitro.Firstly，the ovine ruminal epithelial cells were cultured in vitro，then the epithelial cells were stimulated by Bacillus subtilis with different concentrations at different times，the expression of SBD-1 mRNA was determined by real-time fluorescence quantitative PCR（RT-PCR）.The results indicated that the expression peak of SBD-1 mRNA was after 8 h treated by Bacillus subtilis with 10¹⁰ cfu•mL^-1.The expression of SBD-1 was increased significantly after treated by Bacillus subtilis with different concentrations，and the expression at 10⁹，10¹⁰，10¹¹cfu•mL^-1 showed stronger induction response than the blank（P<0.01）.This study reveals that Bacillus subtilis can induce the expression of SBD-1 in ovine ruminal epithelial cells.

CLC Number:

S826
S813.3

WANG Pei，FAN Yan-ru，JIN Xin，YANG Yin-feng. Effects of Bacillus subtilis on the Expression of SBD-1 in Cultured Ruminal Epithelial Cells of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 760-767.

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Effects of Bacillus subtilis on the Expression of SBD-1 in Cultured Ruminal Epithelial Cells of Sheep

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